Immunofluorescence is the specific antigen and antibody reaction where the antibodies are labeled with a fluorescent dye and the antigen-antibody complex is visualized using fluorescent (UV) microscope. Some of the commonly used fluorochromes include Acridine Orange, Lissamine, Rhodamine, and Calcofluor white. Various factors determine the type of immunofluorescence assay used such as time consumed, cost, complexity, flexibility, sensitivity, cross reactivity and many others. Plus
